Part:BBa_K4179017

8XGTIIC motifs

8XGTIIC is 8 DNA motifs that contain TEA domains which are also called TEAD binding elements [1]. These domains are transcriptional binding sites that allow the TEAD transcription factors to bind to the DNA. YAP/TAZ proteins which are regulatory proteins in the Hippo pathway, translocate to the nucleus and regulate the TEAD proteins by binding to the GTIIC motifs [2].

Usage and Biology

The team of Technion 2022 used this part in a construct (BBa_K4179020) in order to quantify their manufactured metabolite-decursin. These motifs were found in the 8XGTIIC-Luciferase plasmid (#34615 AddGene) upstream to the luciferase gene. It is used as YAP/TAZ responsive Luciferase reporter. It has been proven that their manufactured metabolite affects the Hippo pathway by a cascade of phosphorylation that ends up regulating the TEAD proteins by binding to 8XGTIIC motifs.

References

1.Dupont, S., Morsut, L., Aragona, M., Enzo, E., Giulitti, S., Cordenonsi, M., Zanconato, F., Le Digabel, J., Forcato, M., Bicciato, S., Elvassore, N., & Piccolo, S. (2011). Role of YAP/TAZ in mechanotransduction. Nature, 474(7350), 179–184. https://doi.org/10.1038/nature10137

2.Yuan, P., Hu, Q., He, X., Long, Y., Song, X., Wu, F., He, Y., & Zhou, X. (2020). Laminar flow inhibits the Hippo/YAP pathway via autophagy and SIRT1-mediated deacetylation against atherosclerosis. Cell Death and Disease, 11(2). https://doi.org/10.1038/s41419-020-2343-1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]